Fluo-4 AM
PDP SFA Experience Fragment
Intracellular calcium response of Jurkat cells to stimulation with A23187. Jurkat cells were stained with 5 μM BD Pharmingen™ Fluo-4 AM (Cat. No. 565878) in DPBS with calcium and magnesium for 20 minutes at 37°C. Cells were pelleted once and further incubated in complete medium for 30 minutes to allow complete hydrolysis of AM moieties. Cells were then pelleted and resuspended in DPBS with calcium and magnesium and analyzed on an BD Accuri™ C6 in the FL-1 channel. After 30 seconds of acquisition, 1 μM calcium ionophore A23187 (left, Sigma Aldrich Cat. No. C7522) or DMSO vehicle (right) was added to the tube, followed by mixing with the pipette. Jurkat cells show an increase in intracellular calcium concentration with addition of calcium ionophore A23187, but not with addition of DMSO vehicle.
Fluo-4 AM staining of PC12 cells 3h post-STS treatment depicting
A comparative assessment of fluo Ca2+ indicators in rat ventricular myocytes. - Abstract - Europe PMC
A novel Ca2+ indicator for long-term tracking of intracellular calcium flux
Fluo-4 - an overview ScienceDirect Topics
Mag-Fluo-4 within the ER can reliably measure Ca 2+ uptake and release.
Calcium signaling: breast cancer's approach to manipulation of cellular circuitry
Fluo-4 - an overview ScienceDirect Topics
PhenoVue Fluo-4 AM, Calcium Indicator
Fluo-4 AM
Fluo-4, AM, cell permeant
Good Price CAS:273221-67-3 Fluo-4 AM for Sale - Quotation - ALFA CHEMICAL
Fluo-4 AM Ester - Biotium
