Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination

Scientific Article | Ce protocole décrit en détail la préparation des complexes nucléosomiques à l’aide de deux méthodes de préparation des échantillons
DNA repair in the context of chromatin is poorly understood. Biochemical studies using nucleosome core particles, the fundamental repeating unit of chromatin, show most DNA repair enzymes remove DNA damage at reduced rates as compared to free DNA.

Cryo-EM structure of a licensed DNA replication origin

Cryo-EM reconstruction of NCP (class 1) a, Native gel showing

Structural basis for APE1 processing DNA damage in the nucleosome

IJMS, Free Full-Text

The integrative role of cryo electron microscopy in molecular and

Cryo-EM data processing a, Data processing procedure for the

Chromatin structure meets cryo-EM: Dynamic building blocks of the

3.9 ˚ A phase-plate cryo-EM structure of the Nucleosome Core

Structure and dynamics of the chromatin remodeler ALC1 bound to a

High-resolution structure determination of sub-100 kDa complexes

Polymers, Free Full-Text

JoVE Peer Reviewed Scientific Video Journal - Methods and Protocols

Electron microscopy analysis of ATP-independent nucleosome